Interests & models
My main interests, those I have worked in, are on basic
Neuroscience: cellular physiology, signalling mechanisms and
neurotransmitter receptor function.
The models I have used are mainly:
- Cerebrocortical nerve endings (a.k.a synaptosomes)
- Adrenal chromaffin cells in culture
- Cerebellar Purkinje neurons and granule cells in culture
- Astrocytes in purified cultures from cerebellum
Techniques
- Binding techniques
- [Ca2+]i with probes like Fura-2 or fluo-3 y populations and single cell
- Fluorescent probes imaging
- Software tools for florescence data analysis
- Western blots
- Immunocytochemistry